FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l04n01 mk258.3410000000c3a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l04n01 mk258.3410000000c3a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27236340
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1410080.5177200754580095TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC202150.038.21227629
AGTCACA218200.035.71042328
TCACACT219600.035.3295230
ACACTTG225400.034.31029532
CACACTT232500.033.59189631
CACACGT237650.033.25836612
CAGTCAC234500.033.15028827
CTCGTAT238100.032.29971742
CGTCTGA248800.031.70441216
TCTGAAC253950.031.38040418
GTCTGAA253800.031.26597817
GTATGCC245750.031.17454345
GCACACG254200.031.17332511
CACGTCT254350.031.12760414
CGTATGC247150.031.10736844
ACGTCTG256300.030.81177315
CCAGTCA253650.030.66514226
TCTCGTA251300.030.62997641
TCGTATG254050.030.51983343
CTGAACT260450.030.49358719
Produced by FastQC (version 0.11.2)