FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l04n01 mk257.3410000000c34b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l04n01 mk257.3410000000c34b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8353864
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT16150.019.22501412
AGTCACC18800.015.67822728
CTACTAG7850.014.33124343
GCACACG22750.014.04365111
CCCTAGT1301.916259E-413.856471
CCTAGTC2306.684786E-913.6964222
GCCTAGT2358.954885E-913.4142431
AGCACAC24500.013.22420310
AGGCTAG4300.013.08142643
CCTGAGG8600.013.08072116
ACCTAGG1555.8181715E-513.06454734
CACGTCT25400.012.57809114
CAGATCA21600.012.39586234
CGGGCTT20900.012.2818711
CAGTCAC26000.012.11526827
CGGGACT10550.011.9520261
CCCGGTT17450.011.8713021
ACGTCTG27250.011.64160115
ACACGTC30450.011.37872313
CTTAGGT2402.2461209E-611.2583821
Produced by FastQC (version 0.11.2)