FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l04n01 mk256.3410000000c2ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l04n01 mk256.3410000000c2ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28017648
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1933920.690250659155972TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG277150.037.87988739
CACACGT290100.037.11056512
GTCACGC293550.035.9945929
CAGTCAC298800.035.57951727
TCACGCC295500.035.5514730
AGTCACG299200.035.50256728
GCACACG305700.035.35764311
CACGTCT308850.034.82106814
GCCAATA301550.034.75594334
ACGCCAA306150.034.4028232
ATATCTC307400.034.29134838
CGTCTGA315800.034.14023216
CTCGTAT308150.034.01841442
CACGCCA309350.033.9888831
AGCACAC319850.033.94819610
GTCTGAA321100.033.65379717
ACGTCTG322650.033.394515
CCAATAT316550.033.27935
TCTGAAC324850.033.18219818
CTGAACT324900.033.06628819
Produced by FastQC (version 0.11.2)