FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l04n01 mk255.3410000000c28f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l04n01 mk255.3410000000c28f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20741205
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC387530.18684063920104932TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC63950.031.9126329
CACACAG68200.030.12189531
CACAGTG71150.028.71479833
CACACGT80000.026.88625312
TCACACA77550.026.63524830
AGTCACA80200.025.72679128
CGTCTGA83900.025.42175916
ACGTCTG86350.024.62230715
GCACACG88700.024.40211111
ACACAGT85350.024.06922332
CACGTCT88650.024.05962214
CAGTCAC90450.023.05957627
ACACGTC99200.021.56890513
GTATGCC96400.021.33261545
CGTATGC97250.021.14621444
AGCACAC107100.020.71397410
CTGAACT106800.020.0761719
TCTGAAC109000.019.77412218
TCGTATG108600.019.35086443
CCAGTCA108650.019.09336326
Produced by FastQC (version 0.11.2)