FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l04n01 mk254.3410000000c23f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l04n01 mk254.3410000000c23f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31477555
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC828630.26324471516291525TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGACC136000.032.1465632
AGTCACT143850.031.34571328
CACTGAC154400.028.72381231
GTCACTG159350.028.19854229
CGTCTGA164100.027.96925216
GACCAAT158050.027.50453435
CACACGT166900.027.48654612
ACGTCTG167250.027.40211915
CAGTCAC166150.027.15146627
ACCAATC159850.026.9685336
TCACTGA174250.025.74868430
CACGTCT178550.025.70570814
GCACACG180750.025.48040811
TGACCAA174350.024.86912234
CTGACCA176300.024.81101833
GTATGCC180750.024.36096845
CGTATGC182700.024.15029544
CCAATCT180350.023.91577737
TCTGAAC196800.023.61912518
CCAGTCA191600.023.54489326
Produced by FastQC (version 0.11.2)