FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l04n01 mk253.3410000000c1d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l04n01 mk253.3410000000c1d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33736978
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1288780.3820081336271435TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG165450.042.44470632
CACTTAG172750.040.7557231
CTTAGGC172850.040.34119433
TCACTTA178250.039.46030830
TTAGGCA180950.038.5851134
TAGGCAT203300.034.54181735
AGTCACT208700.034.0042528
AGGCATC228850.030.80186736
CAGTCAC233900.030.48435227
CACACGT237200.030.14400912
CGTCTGA239050.030.00466516
GTATGCC235400.030.00282145
GTCACTT238450.029.81884429
CGTATGC238500.029.58467544
CACGTCT242600.029.48213614
ACGTCTG243850.029.46017815
TCGTATG248300.028.61675543
ACACGTC258500.027.71224613
GCACACG261400.027.46594811
CCAGTCA260150.027.43436626
Produced by FastQC (version 0.11.2)