FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l04n01 mk252.3410000000c14d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l04n01 mk252.3410000000c14d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35636049
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1353990.37994952807478743TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC202500.035.87921528
TATCTCG213250.033.9213339
TGTATCT217000.033.46917737
GTCACCG219750.033.04260329
GTATCTC222550.032.61502538
CAGTCAC232500.031.42282127
ATGTATC231450.031.36949336
CACCGAT234050.031.12955731
ACCGATG233700.031.08943732
TCACCGA235100.030.93310430
CACACGT242800.030.41223312
GTATGCC239000.030.13470545
CGTATGC239800.030.09055344
CGTCTGA246250.030.00438516
CCGATGT243300.029.86272633
CACGTCT253600.029.13477914
CGATGTA249600.029.06386634
ACGTCTG255150.028.96660415
GATGTAT257300.028.71819335
TCGTATG257950.028.23541843
Produced by FastQC (version 0.11.2)