FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l04n01 mk251.3410000000c0c4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l04n01 mk251.3410000000c0c4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11361971
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG3166352.786796410587564TruSeq Adapter, Index 14 (97% over 44bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG248760.21894088622475802TruSeq Adapter, Index 14 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG405100.042.95394515
CACACGT406750.042.9126412
GTCACAG400800.042.67174529
GCACACG410050.042.6062611
CACGTCT408150.042.59988414
AGTCACA403850.042.43268228
CGTCTGA409700.042.35634216
CGTATCT395250.042.34665339
CCGTATC396000.042.09031738
TATCTCG400500.041.9831141
CAGTCAC408150.041.9575227
GTATCTC401750.041.8468840
ACACGTC416550.041.83265313
GTCTGAA415250.041.8281617
AGCACAC418350.041.82549310
TCACAGT408900.041.62835330
TCCGTAT407050.041.25652337
CACAGTT414100.041.18168631
CTGAACT422100.041.07473419
GATCGGA397000.040.9759061
Produced by FastQC (version 0.11.2)