FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l04n01 mk250.3410000000c05b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l04n01 mk250.3410000000c05b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9822440
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC18750.018.72050928
CACACGT21250.017.0462212
GTCACCT20600.016.7121329
ACGTCTG22550.016.3627515
GCACACG26050.014.5099911
CAGTCAC24550.014.29715727
CGTCTGA26750.013.96185816
CACGTCT27550.013.22977214
GTATGCC30100.012.48315645
ACCTTGT29400.012.475018532
GTCTGAA30250.012.42081417
AGCACAC31050.012.390820510
CCCGGTT14200.012.3668571
ACACGTC32000.011.741549513
GCCGTTT33050.011.7168271
TCGTATG34600.011.119904543
CGCGTTT27250.011.0711151
CTCGTAT35250.010.78719742
GTAATCT34050.010.70475537
CGTATGC35750.010.69910144
Produced by FastQC (version 0.11.2)