FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l03n01 ap2_pchx_pn_pd_12.3410000000c4dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l03n01 ap2_pchx_pn_pd_12.3410000000c4dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13289366
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG316490.23815282083434228No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG277200.20858782879484244No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG249270.18757102483293786No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC216330.162784289333291TruSeq Adapter, Index 12 (100% over 51bp)
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC146440.11019336814111375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG30400.031.67697311
CGTATGC30400.031.53025444
GTATGCC31250.030.23982445
AGCACAC35550.027.27791210
TCGTATG35200.027.03890843
ACGTCTG36950.026.18279815
ACACGTC38400.025.25271613
CACACGT38950.024.7228412
CACGTCT39600.024.54429614
CTCGTAT38850.024.49857342
CAGTCAC40500.023.44422127
AGAGCAC41800.023.145458
CGTCTGA42250.022.9515816
GAGCACA42450.022.7910449
TCTCGTA43500.021.82803541
AGTCACC43550.021.6989928
CTGAACT45550.021.53575719
GCTGACT59100.021.0366361
TCGGAAG49200.019.755393
TCCAGTC52150.018.81094425
Produced by FastQC (version 0.11.2)