FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l03n01 ap2_pchx_pn_pd_11.3410000000c49a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l03n01 ap2_pchx_pn_pd_11.3410000000c49a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15195738
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC461560.3037430626929735TruSeq Adapter, Index 11 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG408010.268502918384089No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG329380.21675814626443282No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG321930.21185545578635273No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC197620.1300496231245893No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC155460.1023050015734675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG62100.036.33936311
CGTATGC61950.035.48552744
GTATGCC62100.035.217645
AGCACAC67400.033.7154910
ACGGCTA65700.033.45778332
ACGTCTG68200.033.12129615
AGTCACG67900.032.54026828
CACACGT69600.032.35819212
ACACGTC70000.032.23746513
TCGTATG68550.032.23309343
CACGGCT69250.031.77510831
GCTACAT69250.031.74261935
CACGTCT71850.031.4074114
CTACATC71450.030.5126236
CGTCTGA74050.030.5046916
GGCTACA71950.030.42636134
CAGTCAC73550.030.2241227
CTCGTAT72900.030.03193542
GAGCACA76050.030.028589
AGAGCAC77000.029.7165368