FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l03n01 ap2_pchx_pn_pd_10.3410000000c457.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l03n01 ap2_pchx_pn_pd_10.3410000000c457.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15256146
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG429620.28160454154017667No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG331920.21756477684468936No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC328480.21530994787281138TruSeq Adapter, Index 10 (100% over 51bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG325580.21340907461163522No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC195320.1280270915079077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG45000.034.74805511
GTATGCC45300.033.7760445
CGTATGC47000.032.69894444
AGCACAC50350.031.18991310
ACGTCTG51950.030.05576715
CACTAGC53000.029.46131
TCGTATG52900.029.17959843
ACACGTC54050.028.8463913
TATCTCG52900.028.75386239
CACACGT55750.028.37032912
ACTAGCT55550.028.31111732
CTCGTAT55250.027.93847542
TAGCTTA55300.027.91019434
TCACTAG57400.027.35945330
GTCACTA57300.027.33028229
CACGTCT57400.027.1236514
CGTCTGA58250.027.0754716
GAGCACA58950.026.716069
CAGTCAC59450.026.34180327
TCTCGTA59250.026.31817441
Produced by FastQC (version 0.11.2)