FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l03n01 ap2_pchx_pn_md_09.3410000000c3f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l03n01 ap2_pchx_pn_md_09.3410000000c3f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15893532
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG354020.22274469891274012No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG294610.185364713142428No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG282430.1777012183320863No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC251940.15851731383559048TruSeq Adapter, Index 9 (100% over 51bp)
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC166260.10460859172146254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG37350.032.22766511
GTATGCC38750.030.42634845
CGTATGC39650.029.62398744
AGCACAC43650.027.47315810
AGTCACG43000.027.20901528
ACGTCTG44150.026.90654615
TCGTATG44450.026.5262443
GTCACGA45200.025.7853729
ACACGTC48250.024.8533313
CACACGT48800.024.48116912
CACGTCT48800.024.48101214
CGTCTGA50050.023.91455316
CTCGTAT50400.023.30537842
GAGCACA52450.022.9066419
CAGTCAC51950.022.52141827
AGAGCAC54800.022.1296148
TCTCGTA53650.022.06135741
TCACGAT53650.021.76495730
CTGAACT57200.020.84656719
GCTGACT67700.020.594321
Produced by FastQC (version 0.11.2)