FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l03n01 ap2_pchx_pn_md_08.3410000000c39b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l03n01 ap2_pchx_pn_md_08.3410000000c39b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14930437
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC565340.37864933223320923TruSeq Adapter, Index 8 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG346240.23190212048046552No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG314220.21045599669989565No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG267670.17927807471408908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG69300.038.2456611
GTATGCC70350.037.03667445
CGTATGC70650.036.943644
AGCACAC76000.035.02202210
ACGTCTG76300.034.73618315
TCGTATG75900.034.4178543
CACACGT78200.033.8347812
ACACGTC78850.033.58428613
CACGTCT79800.033.1562814
CTCGTAT78750.033.11511242
CAGTCAC80700.032.62021327
GAGCACA82950.032.1419339
AGAGCAC83700.031.8001658
CGTCTGA83700.031.58448216
TCTCGTA85300.030.75692441
CTGAACT87200.030.31675519
GTCACAC87600.029.99964329
TCCAGTC92800.028.5608925
AGTCACA92100.028.55810228
TCGGAAG93000.028.5473773
Produced by FastQC (version 0.11.2)