FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l03n01 ap2_pchx_pn_md_07.3410000000c33e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l03n01 ap2_pchx_pn_md_07.3410000000c33e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16937909
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG385080.22734801562577767No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG331780.19588014081313104No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG317050.1871836718452083No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC170750.10080937381349729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG26600.027.91246811
CGTATGC29350.024.30297344
GTATGCC29350.024.2258845
AGCACAC32500.022.84521310
ACGTCTG32500.022.42931615
GCTGACT72850.021.0536441
CACACGT35850.020.52180312
TCGTATG35200.020.51968243
ACACGTC37150.019.86412413
CTCGTAT35800.019.67295342
CACGTCT39800.018.48498314
GAGCACA41200.018.239549
AGAGCAC41600.017.8478228
CTAGCAG86500.017.8431978
ACTCTAG90800.017.3946085
CTGACTC90350.017.0825082
CGTCTGA42650.017.03875516
CAGTCAC43150.016.73837327
GACTCTA95850.016.689064
CATCTCG43100.016.60176539
Produced by FastQC (version 0.11.2)