FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l03n01 ap2_mchx_pn_pd_06.3410000000c2df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l03n01 ap2_mchx_pn_pd_06.3410000000c2df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17740954
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC686990.38723396723761305TruSeq Adapter, Index 6 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG520570.2934284142780597No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG417150.23513391669918088No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG400250.2256079351764285No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC214580.12095178196166903No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC197190.1111496033415114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG82000.038.5503411
CGTATGC83300.037.2764144
CACACGT86550.036.62718212
GTATGCC85800.036.3727145
ACACGTC88000.035.94696813
ACGTCTG88500.035.84556615
CACGCCA87200.035.58061631
GTCACGC89000.034.9635129
AGTCACG89500.034.91892228
TCACGCC88950.034.85531230
TCGTATG89700.034.7923743
CACGTCT91750.034.649414
CGCCAAT89550.034.62177733
AGCACAC92950.034.29937410
TATCTCG92400.033.67811239
CTCGTAT94550.032.88868342
ACGCCAA95800.032.55093432
GCCAATA97850.031.77734
CGTCTGA99900.031.7325616
TCTCGTA98750.031.48987441