Basic Statistics
Measure | Value |
---|---|
Filename | C5WCJACXX l03n01 ap2_mchx_pn_pd_05.3410000000c272.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 16685985 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG | 48516 | 0.290758981264816 | No Hit |
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG | 40615 | 0.24340786594258595 | No Hit |
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG | 37618 | 0.22544668474770896 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC | 36608 | 0.2193937007614474 | TruSeq Adapter, Index 5 (100% over 51bp) |
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC | 21358 | 0.12799963562235012 | No Hit |
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC | 17290 | 0.10361989418065522 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCACACG | 5010 | 0.0 | 33.68137 | 11 |
CGTATGC | 5025 | 0.0 | 32.91217 | 44 |
ACGTCTG | 5355 | 0.0 | 31.552773 | 15 |
ACACGTC | 5330 | 0.0 | 31.531923 | 13 |
GTATGCC | 5305 | 0.0 | 31.343676 | 45 |
CACACGT | 5435 | 0.0 | 31.005726 | 12 |
AGCACAC | 5585 | 0.0 | 30.294294 | 10 |
TCGTATG | 5545 | 0.0 | 29.988043 | 43 |
CACGTCT | 5715 | 0.0 | 29.486462 | 14 |
CTCGTAT | 5840 | 0.0 | 28.434706 | 42 |
CACAGTG | 6075 | 0.0 | 27.369097 | 33 |
ACACAGT | 6085 | 0.0 | 27.17622 | 32 |
GTCACAC | 6160 | 0.0 | 26.773333 | 29 |
CACACAG | 6315 | 0.0 | 26.364565 | 31 |
CAGTCAC | 6395 | 0.0 | 26.141161 | 27 |
TCTCGTA | 6435 | 0.0 | 25.770578 | 41 |
CGTCTGA | 6760 | 0.0 | 25.094683 | 16 |
GAGCACA | 6905 | 0.0 | 24.405308 | 9 |
AGAGCAC | 7185 | 0.0 | 23.830002 | 8 |
CTGAACT | 7220 | 0.0 | 23.308882 | 19 |