FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l03n01 ap2_mchx_pn_pd_05.3410000000c272.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l03n01 ap2_mchx_pn_pd_05.3410000000c272.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16685985
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG485160.290758981264816No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG406150.24340786594258595No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG376180.22544668474770896No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC366080.2193937007614474TruSeq Adapter, Index 5 (100% over 51bp)
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC213580.12799963562235012No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC172900.10361989418065522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG50100.033.6813711
CGTATGC50250.032.9121744
ACGTCTG53550.031.55277315
ACACGTC53300.031.53192313
GTATGCC53050.031.34367645
CACACGT54350.031.00572612
AGCACAC55850.030.29429410
TCGTATG55450.029.98804343
CACGTCT57150.029.48646214
CTCGTAT58400.028.43470642
CACAGTG60750.027.36909733
ACACAGT60850.027.1762232
GTCACAC61600.026.77333329
CACACAG63150.026.36456531
CAGTCAC63950.026.14116127
TCTCGTA64350.025.77057841
CGTCTGA67600.025.09468316
GAGCACA69050.024.4053089
AGAGCAC71850.023.8300028
CTGAACT72200.023.30888219