Basic Statistics
Measure | Value |
---|---|
Filename | C5WCJACXX l03n01 ap2_mchx_pn_pd_04.3410000000c208.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 19351827 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC | 96785 | 0.5001336566309734 | TruSeq Adapter, Index 4 (100% over 51bp) |
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG | 43996 | 0.2273480431589224 | No Hit |
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG | 38112 | 0.19694264526031574 | No Hit |
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG | 34629 | 0.1789443446347469 | No Hit |
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT | 22288 | 0.11517258809723753 | No Hit |
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC | 19361 | 0.10004740120919849 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCACACG | 12040 | 0.0 | 39.411144 | 11 |
CGTATGC | 11875 | 0.0 | 39.279613 | 44 |
GTATGCC | 12035 | 0.0 | 38.850082 | 45 |
CACACGT | 12435 | 0.0 | 38.285305 | 12 |
ACGTCTG | 12480 | 0.0 | 38.074947 | 15 |
ACACGTC | 12500 | 0.0 | 38.014027 | 13 |
ACTGACC | 12420 | 0.0 | 37.697903 | 32 |
TCGTATG | 12680 | 0.0 | 36.87464 | 43 |
CTCGTAT | 12655 | 0.0 | 36.751904 | 42 |
CACGTCT | 12990 | 0.0 | 36.718647 | 14 |
AGCACAC | 13210 | 0.0 | 36.005688 | 10 |
CACTGAC | 13270 | 0.0 | 35.41883 | 31 |
TCTCGTA | 13380 | 0.0 | 34.87821 | 41 |
CGTCTGA | 13775 | 0.0 | 34.544487 | 16 |
AGTCACT | 13800 | 0.0 | 34.07564 | 28 |
CTGAACT | 14060 | 0.0 | 33.796253 | 19 |
CAGTCAC | 13930 | 0.0 | 33.709175 | 27 |
GAGCACA | 14540 | 0.0 | 32.897873 | 9 |
CTGACCA | 14395 | 0.0 | 32.619514 | 33 |
AGAGCAC | 14720 | 0.0 | 32.5873 | 8 |