FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l03n01 ap2_mchx_pn_pd_04.3410000000c208.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l03n01 ap2_mchx_pn_pd_04.3410000000c208.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19351827
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC967850.5001336566309734TruSeq Adapter, Index 4 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG439960.2273480431589224No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG381120.19694264526031574No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG346290.1789443446347469No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT222880.11517258809723753No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC193610.10004740120919849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG120400.039.41114411
CGTATGC118750.039.27961344
GTATGCC120350.038.85008245
CACACGT124350.038.28530512
ACGTCTG124800.038.07494715
ACACGTC125000.038.01402713
ACTGACC124200.037.69790332
TCGTATG126800.036.8746443
CTCGTAT126550.036.75190442
CACGTCT129900.036.71864714
AGCACAC132100.036.00568810
CACTGAC132700.035.4188331
TCTCGTA133800.034.8782141
CGTCTGA137750.034.54448716
AGTCACT138000.034.0756428
CTGAACT140600.033.79625319
CAGTCAC139300.033.70917527
GAGCACA145400.032.8978739
CTGACCA143950.032.61951433
AGAGCAC147200.032.58738