FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l03n01 ap2_mchx_pn_md_03.3410000000c19d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l03n01 ap2_mchx_pn_md_03.3410000000c19d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15539549
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC419470.2699370490095948TruSeq Adapter, Index 3 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG316580.20372534621178515No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG274420.17659457169574225No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG267350.17204489010588403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG55050.035.8844911
CGTATGC55300.035.3989444
ACTTAGG58450.034.0662432
GTATGCC57550.034.0534145
ACACGTC59050.033.6051413
CACACGT58850.033.60508712
ACGTCTG59900.032.9780315
CACTTAG60250.032.93646231
TCGTATG62150.031.78699943
CACGTCT63050.031.43748714
AGCACAC64850.030.60047710
CTCGTAT64700.030.56896442
TAGGCAT65350.030.09062835
TCACTTA67200.029.6305330
TTAGGCA67550.029.11062234
CATCTCG69650.028.5900939
CGTCTGA70450.028.45468316
TCTCGTA70150.028.19404241
AGTCACT71150.028.11312128
CAGTCAC71950.027.61290427
Produced by FastQC (version 0.11.2)