FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l03n01 ap2_mchx_pn_md_02.3410000000c109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l03n01 ap2_mchx_pn_md_02.3410000000c109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17408102
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC714030.4101710801097098TruSeq Adapter, Index 2 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG399840.22968615418269034No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG338960.19471393262746278No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG318280.18283440664582504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG86800.038.1819811
CGTATGC85950.038.01159744
GTATGCC88350.036.9270345
ACGTCTG91750.036.2189715
ACACGTC91600.036.1554713
CACACGT92400.035.769812
TCGTATG93850.035.02767643
CACGTCT96200.034.473414
AGCACAC97500.034.24559810
CTCGTAT95950.033.95619242
TATCTCG96650.033.68659239
CGATGTA97500.033.4601334
TCTCGTA102000.032.0965441
CCGATGT103500.031.71605333
CGTCTGA105750.031.55167816
AGTCACC104700.031.39615428
CAGTCAC106200.031.01626627
GAGCACA107800.030.994399
ATGTATC107850.030.47837336
AGAGCAC110750.030.1891258
Produced by FastQC (version 0.11.2)