FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l03n01 ap2_mchx_pn_md_01.3410000000c09e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l03n01 ap2_mchx_pn_md_01.3410000000c09e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17013775
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG361270.2123397070902842No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG311400.18302816394362803No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG307460.18071239334010236No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC171700.10091822655465937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT79750.019.1213631
ACTCTAG86900.017.7351515
GACTCTA90900.017.1277334
GCACACG22250.016.78580511
CTGACTC91100.016.719832
CTAGCAG92050.016.7184168
CGTATGC21100.016.20936844
GTATGCC21400.016.19199645
ACGTCTG25550.014.70557515
TAGCAGA105050.014.60666759
CTCTAGC106400.014.5482976
ACACGTC26250.014.39913613
CACACGT27800.013.51541512
CGCGGGG6500.013.5148771
TGACTCT118100.013.4117153
CTATCGA113150.013.302299517
TCTATCG112850.013.15827116
TCGTATG27750.012.97364243
GATCTAT121750.012.39966614
AGATCTA123250.012.37653813
Produced by FastQC (version 0.11.2)