FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l02n01 ap2_pchx_mn_pd_24.3410000000c4ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l02n01 ap2_pchx_mn_pd_24.3410000000c4ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14264147
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG440130.308556831333833No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG287160.20131592867067338No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG281790.19755124509022517No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC263260.1845606330332967TruSeq Adapter, Index 12 (100% over 51bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA238010.1668589085628464No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC191110.13397926984347538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG36900.033.4138611
GTATGCC37200.032.9639945
CGTATGC37750.032.3657644
AGCACAC42450.029.5752910
TCGTATG42600.028.73374743
ACGTCTG44800.027.72218915
ACACGTC46000.026.94999313
CACACGT46650.026.86414312
CTCGTAT47500.025.86310442
AGAGCAC50300.025.2726888
CACGTCT49550.025.06457314
GAGCACA50400.024.9547829
CGTCTGA50300.024.73557516
GCTGACT53200.024.3073671
CAGTCAC50950.024.11089727
TCTCGTA51800.023.62929341
AGTCACC53950.022.89527128
CTGAACT55000.022.78543919
TCGGAAG61100.020.5841983
TCCAGTC60400.020.45025625
Produced by FastQC (version 0.11.2)