FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l02n01 ap2_pchx_mn_pd_23.3410000000c4a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l02n01 ap2_pchx_mn_pd_23.3410000000c4a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10641048
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG272440.2560274138411931No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG200750.18865623010064422No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG185610.1744283081891934No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC175460.1648897740147399TruSeq Adapter, Index 11 (100% over 51bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA153780.14451584092093187No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC125430.11787372822676864No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT107300.1008359327013655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG24900.033.1617711
GTATGCC26200.030.31507945
CGTATGC26350.029.97214944
ACGTCTG28850.028.3093415
AGCACAC29100.028.29835110
ACGGCTA29450.027.19766632
TCGTATG29550.026.6502943
CACACGT30950.026.38851212
ACACGTC31050.026.37598813
AGTCACG30550.026.07107728
CACGTCT31700.025.76417714
CACGGCT31450.025.39654531
GCTGACT41300.024.6574131
GGCTACA32500.024.36824234
GAGCACA34150.024.2454439
CAGTCAC33250.024.08936127
CTCGTAT32250.024.06935542
CGGCTAC34000.023.88884733
CTACATC33500.023.84231436
GCTACAT32800.023.80238735
Produced by FastQC (version 0.11.2)