FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l02n01 ap2_pchx_mn_pd_22.3410000000c463.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l02n01 ap2_pchx_mn_pd_22.3410000000c463.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10481458
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG299460.2857045269847No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG212900.20312059639031135No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG202850.19353223568705805No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA171060.16320248576104585No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC161450.15403391398410413TruSeq Adapter, Index 10 (100% over 51bp)
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC133300.1271769633575787No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT111800.10666454991280794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG23800.032.3309511
GTATGCC23700.031.61583145
CGTATGC24750.030.45725444
AGCACAC27350.028.38122210
TCGTATG27950.027.13121443
ACGTCTG28200.026.9668815
CACACGT30250.025.43700212
CACTAGC29700.025.37765531
TATCTCG30100.025.266139
GCTGACT41650.024.9888151
ACACGTC31400.024.21866413
GAGCACA32850.024.0403699
CACGTCT31950.023.94259314
TAGCTTA31500.023.9273934
ACTAGCT31400.023.860432
CTCGTAT32800.023.32346542
CAGTCAC32900.023.04619227
TCACTAG32950.022.94283130
AGTCACT33300.022.70179728
CGTCTGA34150.022.59771316
Produced by FastQC (version 0.11.2)