FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l02n01 ap2_pchx_mn_md_21.3410000000c406.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l02n01 ap2_pchx_mn_md_21.3410000000c406.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10949948
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG236820.21627499966209887No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC222720.20339822618335723TruSeq Adapter, Index 9 (100% over 51bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG173450.1584025787154423No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG161020.14705092663453745No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA143840.13136135441008487No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC111880.10217400119160384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG32850.034.17729611
CGTATGC33100.033.24184444
GTATGCC34350.032.293645
AGCACAC37700.030.07888210
CACACGT38950.029.0556812
AGTCACG38300.029.01982128
ACGTCTG38750.028.9732615
TCGTATG38050.028.91734743
GTCACGA38450.028.73106229
CACGTCT40350.027.65710314
ACACGTC40800.027.4073313
CTCGTAT40850.027.09876642
GAGCACA43150.026.227679
AGAGCAC43550.025.9865348
CAGTCAC43050.025.81786527
TCTCGTA44100.025.35678941
TCACGAT43750.025.35335530
CGTCTGA44750.024.98779316
TCCAGTC47050.024.05320525
CTGAACT47700.023.34809119
Produced by FastQC (version 0.11.2)