FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l02n01 ap2_pchx_mn_md_20.3410000000c3b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l02n01 ap2_pchx_mn_md_20.3410000000c3b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14728882
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG306670.20820996461238536No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC302890.20564357837886135TruSeq Adapter, Index 8 (100% over 51bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG244570.16604790506163333No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG210730.14307263782818005No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA177630.12059978483091928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG40450.034.59732411
CGTATGC41050.033.54604744
GTATGCC41550.033.41256745
AGCACAC45800.030.60505710
ACGTCTG49150.028.61031715
TCGTATG49000.028.42481443
ACACGTC51400.027.44555913
CACACGT51250.027.4381812
CACGTCT51900.027.18105914
CTCGTAT53350.026.06319442
GAGCACA55000.025.6902059
CAGTCAC55100.025.3983227
CGTCTGA55850.025.25858916
AGAGCAC57350.024.6374288
GTCACAC58100.024.24177429
CTGAACT58350.024.0607119
TCTCGTA58100.023.97110741
GCTGACT53700.023.4519561
TCCAGTC64350.022.0270725
AGTCACA65000.021.66841728
Produced by FastQC (version 0.11.2)