FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l02n01 ap2_pchx_mn_md_19.3410000000c364.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l02n01 ap2_pchx_mn_md_19.3410000000c364.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13794616
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG308770.22383370439597594No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG236070.17113198366667112No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG227140.16465844355507975No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC201070.1457597659840622TruSeq Adapter, Index 7 (100% over 51bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA179890.1304059496835577No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC145010.10512072246157488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG30150.031.267911
GTATGCC30850.030.1220345
CGTATGC30800.030.09842144
AGCACAC35300.026.64241810
ACGTCTG35600.026.29121415
ACACGTC36900.025.36496413
TCGTATG37350.024.94060343
CACACGT38400.024.4913312
GAGCACA41200.023.4278349
CACGTCT40900.022.9392114
CTCGTAT40300.022.83476842
CGTCTGA42900.021.97467416
AGAGCAC44500.021.6904098
GCTGACT50300.021.6323661
CAGTCAC43800.021.266327
CATCTCG44350.021.20610839
AGTCACC45200.020.6076128
TCTCGTA46650.020.54642541
CTGAACT45650.020.40446919
TCGGAAG53200.018.1855183
Produced by FastQC (version 0.11.2)