FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l02n01 ap2_mchx_mn_pd_18.3410000000c307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l02n01 ap2_mchx_mn_pd_18.3410000000c307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10914895
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG305880.2802409001644084No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC245550.22496780775261696TruSeq Adapter, Index 6 (100% over 51bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG235670.21591595704768574No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG216670.198508551845895No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA165230.15138029271009937No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT142370.13043643571468164No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC141970.1300699640262229No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC117460.10761441131591279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG32000.033.81900411
CGTATGC32350.032.34358644
ACGTCTG33500.031.9017515
GTATGCC33250.031.46725545
CACACGT35350.030.4866612
CACGCCA34600.030.36718231
AGTCACG34750.030.36559128
TCGTATG35050.030.04466243
AGCACAC36250.029.97815110
ACACGTC36600.029.38397413
GTCACGC36200.029.21144529
TCACGCC36350.029.09090230
CGCCAAT36450.029.01109133
CTCGTAT36700.028.50811842
CACGTCT38050.028.20508814
TATCTCG37150.028.16279639
GCCAATA38900.026.95256234
ACGCCAA39850.026.47940632
TCTCGTA41150.025.37054441
CGTCTGA42650.025.11040316