FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l02n01 ap2_mchx_mn_pd_17.3410000000c29c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l02n01 ap2_mchx_mn_pd_17.3410000000c29c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11438544
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG319460.27928379695877376No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC245840.21492245866257106TruSeq Adapter, Index 5 (100% over 51bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG242720.21219483878367737No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG225560.19719292944976213No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA168250.14709039891790424No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC147410.12887129690631954No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT133300.11653581085145104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG35000.034.19870811
CGTATGC35050.033.8958244
ACGTCTG36550.033.1793215
CACACGT37000.032.6541712
GTATGCC36750.032.5721945
ACACGTC39200.030.99372713
TCGTATG40150.029.8144243
AGCACAC40600.029.75872810
CACGTCT41500.029.49273114
CTCGTAT43100.027.459242
CACAGTG44300.027.12075433
ACACAGT44300.027.06996532
CGTCTGA45350.026.7906116
GTCACAC44650.026.70671829
CACACAG44950.026.62846831
TCTCGTA45700.026.38930541
CAGTCAC46250.025.83145527
GAGCACA47650.025.4030289
CTGAACT50300.024.24361619
AGTGATC50800.023.78344536
Produced by FastQC (version 0.11.2)