FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l02n01 ap2_mchx_mn_pd_16.3410000000c222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l02n01 ap2_mchx_mn_pd_16.3410000000c222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12502400
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG356980.28552917839774766No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG271310.2170063347837215No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG258770.20697626055797289No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC251010.2007694522651651TruSeq Adapter, Index 4 (100% over 51bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA191140.1528826465318659No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC161480.1291592014333248No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT139770.11179453544919375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG35350.035.2605211
CGTATGC37550.032.11895844
GTATGCC38300.031.66510845
CACACGT40350.031.11410512
ACGTCTG40000.031.10486415
ACACGTC41250.030.21695713
ACTGACC41250.029.50801332
TCGTATG41450.029.25976643
AGCACAC43600.029.00146710
CACGTCT44350.028.05399114
CTCGTAT43900.027.93256242
CACTGAC44650.027.41221231
CGTCTGA48600.025.64690416
AGTCACT48500.025.37536228
CAGTCAC48750.025.24523227
TCTCGTA49050.024.95391541
CTGAACT51450.024.0513119
GAGCACA53350.023.9121599
GTCACTG53150.023.19764729
CTGACCA53750.022.89683733