FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l02n01 ap2_mchx_mn_md_15.3410000000c1b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l02n01 ap2_mchx_mn_md_15.3410000000c1b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11108046
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC235480.21199047969372833TruSeq Adapter, Index 3 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG232880.2096498340032081No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG198340.17855525625298996No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG137680.12394619179646899No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA131280.11818460240441929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG33050.033.3573211
CGTATGC33500.032.6430944
ACTTAGG33400.032.4690132
GTATGCC33650.032.2965445
TCGTATG36250.030.29085743
CACTTAG36050.030.2070731
ACGTCTG36450.030.12221115
ACACGTC36550.030.03979513
CACACGT37250.029.59609212
CACGTCT39650.027.7479114
AGCACAC39750.027.73482510
TAGGCAT39150.027.70000535
TCACTTA40500.026.99912830
TTAGGCA40600.026.87696634
CTCGTAT40950.026.70303542
TCTCGTA42100.026.1339541
CATCTCG43700.025.69185439
CGTCTGA43450.025.37294616
AGGCATC43750.025.09610636
AGTCACT44900.024.704128
Produced by FastQC (version 0.11.2)