FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l02n01 ap2_mchx_mn_md_14.3410000000c123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l02n01 ap2_mchx_mn_md_14.3410000000c123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14167350
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC412350.2910565490370465TruSeq Adapter, Index 2 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG312880.22084581802524816No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG243550.17190935496052545No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG221700.15648656947135492No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA168110.11866015874528404No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC142460.10055514969277952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG54600.037.04540611
CGTATGC54900.035.86180544
GTATGCC57300.034.5165745
ACACGTC59050.034.17746713
ACGTCTG59450.034.13673815
CACACGT59900.033.73003812
TCGTATG61150.032.4908243
AGCACAC62750.032.341510
CACGTCT62550.032.33688414
CTCGTAT62900.031.5851942
TATCTCG63300.031.2078839
CGATGTA64550.030.77728334
CCGATGT68550.028.91573533
AGTCACC69800.028.75247428
TCTCGTA69600.028.60931641
CAGTCAC70750.028.0801927
CGTCTGA72700.027.85322216
GAGCACA73700.027.8416339
GATGTAT73900.027.09638435
ATGTATC74400.026.85380636