FastQCFastQC Report
Thu 28 May 2015
C5WCJACXX l02n01 ap2_mchx_mn_md_13.3410000000c0ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5WCJACXX l02n01 ap2_mchx_mn_md_13.3410000000c0ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13328927
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG372670.2795948991242881No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG306960.23029610710599582No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG267330.2005637813156303No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA203000.1523003314520366No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC175660.13178855282199384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT61450.020.4210531
CTGACTC65400.019.231072
ACTCTAG67800.018.8489155
CTAGCAG69650.017.9929288
GACTCTA72350.017.8810144
CTCTAGC80800.015.8441386
TAGCAGA80100.015.7018319
TCTATCG88150.014.01246416
CTATCGA88250.013.99658617
ATCTATC95550.012.85670715
TGACTCT102500.012.8408813
GATCTAT95850.012.81641814
AGATCTA97300.012.810410513
GCAGATC104200.011.962255511
TATCGAT104650.011.88913918
CTAGCAT107050.011.32834826
TCTAGCA160200.011.1513067
TTCTAGC112900.010.98050124
AGCAGAT115200.010.87861710
GCATGAC113900.010.82483629
Produced by FastQC (version 0.11.2)