FastQCFastQC Report
Wed 4 Feb 2015
lane6_Undetermined_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane6_Undetermined_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4800336
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC78920.16440515830558527TruSeq Adapter, Index 11 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCCGTCCCGATCTCGTAT65610.13667793254472188TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG100750.042.5352111
ACACGTC100600.042.23988713
CACACGT101250.042.2137112
ACGTCTG101000.041.7844315
CACGTCT101650.041.71546614
CGTCTGA101050.041.67513316
GATCGGA103250.041.351571
AGCACAC104400.041.1338910
AGAGCAC106450.040.5735248
GAACTCC101600.040.53713621
TCGGAAG108000.040.4211773
CTCCAGT96150.040.24326324
CAGTCAC76250.039.99246627
CCAGTCA87700.039.87000726
CGGAAGA109900.039.797024
CTGAACT105500.039.46798319
TGAACTC106450.039.04884320
ACTCCAG102000.038.8399523
TCCAGTC95950.038.5787925
ATCGGAA112850.038.3262942
Produced by FastQC (version 0.11.2)