FastQCFastQC Report
Wed 4 Feb 2015
lane4_Undetermined_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane4_Undetermined_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5182917
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC106900.20625450880266846TruSeq Adapter, Index 1 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC77190.14893157656200168TruSeq Adapter, Index 4 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC65720.12680118165118215TruSeq Adapter, Index 2 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTGACCAATCTCGTATGCC55640.10735267417942446TruSeq Adapter, Index 4 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG398350.044.0478111
ACACGTC397900.043.8354313
CACGTCT397500.043.80835714
CACACGT399750.043.7870212
ACGTCTG399100.043.5762915
GATCGGA410200.043.507341
AGCACAC404900.043.46256310
TCGGAAG411450.043.40423
AGAGCAC408650.043.39778
CGTCTGA400900.043.36458616
CGGAAGA412950.043.284574
ATCGGAA415100.043.0483322
GAACTCC398100.042.98116321
GAGCACA413950.042.7569
CTCCAGT375650.042.7310524
TGAACTC404100.042.61910620
GAAGAGC419500.042.5704776
CCAGTCA357900.042.55738426
CTGAACT405400.042.54773319
TCTGAAC407500.042.40381218
Produced by FastQC (version 0.11.2)