FastQCFastQC Report
Wed 4 Feb 2015
lane3_Undetermined_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane3_Undetermined_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5455452
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGAATTCTCCAGTTCCGCAAGCGACGACCAAGACGGCGCAATCGGCCTG213540.3914249451741121No Hit
GCGATCAGGATACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGC90900.16662230737251468No Hit
CAGGATACTAGAACCGAGGTCCAAGCACACACCAGAAAGCGTCCGCAAGCA87940.16119654246797516No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGAT82150.15058330638781167No Hit
CCGGCCTCGAATTCTCCAGTTCCGCAAGCGACGACCAAGACGGCGCAATCG70270.1288069256222949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT58800.041.11519212
CGTCTGA60300.039.7008616
GCACACG62000.039.50225411
ACGTCTG61350.039.2033815
CACGTCT63300.037.8545814
ACACGTC63900.037.7074413
CTGAACT63350.037.03612519
CAGTCAC42600.036.1548127
GTCTGAA66800.035.80431717
GATCGGA63500.035.3928031
CCAGTCA54300.034.86215626
CGGAAGA72350.034.5931364
TCTGAAC70900.033.42191718
ATCGGAA68200.032.870032
GAGCACA76750.032.232079
ACTCCAG68950.031.63899423
AACTCCA71750.031.50538622
GAACTCC74700.031.21394321
TGAACTC76650.030.54826220
TCCAGTC65900.030.37291325
Produced by FastQC (version 0.11.2)