FastQCFastQC Report
Wed 4 Feb 2015
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16086061
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC2037831.2668297105176962TruSeq Adapter, Index 8 (100% over 51bp)
GTATGCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTG278310.1730131447344381No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTC231230.14374556953377213No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGCATCTCGTATGCCG214590.13340120990464974TruSeq Adapter, Index 20 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGCATCTCGTATGCCG196050.12187570344287517TruSeq Adapter, Index 2 (97% over 36bp)
ATGCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGC164750.10241786351549954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT754550.043.96949412
GCACACG758850.043.73810211
CGTCTGA757150.043.51262316
ACGTCTG768100.043.00633615
CACGTCT770800.042.8935714
GTCTGAA769450.042.814817
CTGAACT765850.042.8098419
ACACGTC773950.042.7948113
GATCGGA720650.042.7629931
TCTGAAC774100.042.4314118
GAGCACA791450.042.2255369
CGGAAGA756200.041.957324
CAGTCAC696400.041.7253227
ATCGGAA745000.041.602462
AGCACAC802800.041.5109410
TCCAGTC746850.040.9172425
ACACTTG259800.040.79808832
AGAGCAC822600.040.6647728
AAGAGCA836000.040.2788967
AACTCCA801950.040.06290422
Produced by FastQC (version 0.11.2)