FastQCFastQC Report
Wed 4 Feb 2015
SE376_ATCACG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE376_ATCACG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8147912
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC103410.12691595098228847TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG20450.029.81141511
CACACGT21700.028.09416412
ACACGTC22250.027.09555413
GTATGCC20500.026.13197745
ACGTCTG24250.025.13916215
CGTATGC21150.025.00959444
CATCACG21550.024.64423233
CACGATC21450.024.23426636
AGCACAC26250.023.3102110
CAGTCAC25000.022.94781927
CACGTCT26750.022.53742414
TCGTATG24050.022.2746643
CTCGTAT24050.022.1806642
ACGATCT24300.021.66899537
CGTCTGA28950.020.9024416
TCACGAT26050.020.4737135
AGAGCAC31350.020.092558
TCTCGTA27550.019.9333441
CCAGTCA30100.019.80708726
CGATCTC26850.019.77866238
Produced by FastQC (version 0.11.2)