FastQCFastQC Report
Wed 4 Feb 2015
SE375_GGTAGC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE375_GGTAGC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14703165
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC755890.5141001954341123TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA94050.039.23534831
GTCACGG97550.038.67973329
AGTCACG98300.038.4742128
GGTAGCA94450.038.26246634
CGGTAGC95200.038.17324433
GCACACG102550.037.7980811
CGTATGC95900.037.7162644
GTATGCC98050.037.2565245
ACGTCTG104750.037.046315
ACACGTC104800.036.9427613
CACGTCT106200.036.51930614
CACACGT106700.036.34891512
TCACGGT102500.036.2214530
GTAGCAT99850.036.17065435
CTCGTAT101600.035.59907542
CGTCTGA109150.035.3261916
TAGCATC102100.035.30707636
CAGTCAC108500.035.1266127
TCGTATG104200.034.79839343
CCAGTCA113250.033.89170526
Produced by FastQC (version 0.11.2)