FastQCFastQC Report
Wed 4 Feb 2015
SE374_GAGTGG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE374_GAGTGG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12804163
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC281500.219850372101636TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG41750.032.44122328
CGTATGC42350.031.35806344
GCACACG45750.031.17571311
ACGTCTG47200.030.45546315
GTATGCC44250.030.0624845
ACGAGTG45100.029.9846732
CGAGTGG44700.029.95189933
ACACGTC48150.029.94813513
GTCACGA45400.029.83516129
CACACGT48900.029.3513712
CACGTCT49800.029.00093714
CACGAGT48200.027.96239331
CTCGTAT47700.027.88662542
TCGTATG49300.027.30265443
CAGTCAC51400.026.7882227
CGTCTGA54600.026.32769816
TCACGAG52950.025.83711630
GGATCTC51450.025.80357238
GATCTCG51400.025.69794339
AGCACAC56900.025.65964510
Produced by FastQC (version 0.11.2)