FastQCFastQC Report
Wed 4 Feb 2015
SE373_CGTACG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE373_CGTACG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14836466
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC337260.22731828455644357TruSeq Adapter, Index 22 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG42300.036.9712833
CGTACGA44200.035.12794534
GTCACCG50850.031.59362629
GCACACG54950.031.23789411
CGTATGC50750.030.77974544
ACACGTC56300.030.48792613
CACCGTA52650.029.8732731
ACGTCTG56950.029.82383715
AGTCACC54650.029.6422528
GTATGCC52950.029.58590545
GTACGAT52600.029.56073835
CACACGT58550.029.20193512
TACGATC53450.029.13156336
CACGTCT59700.028.45004314
CAGTCAC59150.027.76685527
CGATCTC56900.027.5230538
ACCGTAC57100.027.42701532
TCGTATG57900.027.36744143
CGTCTGA62650.027.28995316
GATCTCG57600.027.15032639
Produced by FastQC (version 0.11.2)