FastQCFastQC Report
Wed 4 Feb 2015
SE372_GTGGCC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE372_GTGGCC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7062941
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC1919582.7178196731361624TruSeq Adapter, Index 20 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT225600.043.26861612
GCACACG226700.043.0887411
ACACGTC227100.042.98191513
AGTCACG223750.042.73605728
TCACGTG220600.042.71848730
GTATGCC218500.042.715945
GTCACGT222150.042.60003329
ACGTCTG230000.042.4399715
GTGGCCA218350.042.39639334
CACGTCT230700.042.30144514
CGTGGCC219950.042.26159733
AGCACAC231850.042.23836510
CGTATGC220650.042.1874844
CGTCTGA233200.041.88654316
CAGTCAC229900.041.85707527
TCGTATG223000.041.83373643
CACGTGG225100.041.73395531
CTCGTAT223500.041.65870342
TGGCCAT222900.041.6117235
AGAGCAC237000.041.4537128