FastQCFastQC Report
Wed 4 Feb 2015
SE371_GTGAAA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE371_GTGAAA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14706632
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG4148832.82106059361518TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG506800.042.8707411
AGTCACG500400.042.76312628
ACACGTC505000.042.64848313
GTCACGT499300.042.58042529
TCGTATG501150.042.53270745
ACGTCTG505800.042.5009715
CACACGT510950.042.4782212
CACGTCT506750.042.43017214
CGTCTGA508500.042.23106416
CTCGTAT498150.042.1561444
CAGTCAC509100.041.90361827
GATCTCG498450.041.87856341
CGATCTC488200.041.8772240
AGCACAC519950.041.8730310
TCACGTG507700.041.8297330
ACGATCT488400.041.8171839
CACGTGA509100.041.76360731
GAACTCC512700.041.71668221
CCAGTCA511600.041.59770626
TCCAGTC512850.041.54004725
Produced by FastQC (version 0.11.2)