FastQCFastQC Report
Wed 4 Feb 2015
SE370_GTCCGC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE370_GTCCGC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16274774
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG8218235.049673808066397TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG989200.043.74880611
ACACGTC991300.043.62141813
CACACGT992700.043.59002712
TCGTATG986700.043.46860545
CGTCCGC966650.043.37684233
ACGTCCG972250.043.364932
GATCGGA990350.043.345251
GTCCGCA966400.043.32559234
AGTCACG991050.043.31528528
TCCGCAC963600.043.22730635
CACGTCT998250.043.22532314
CACGTCC975050.043.22177531
CGCACAT972950.043.22032537
GTCACGT988150.043.2078729
CGTCTGA1000800.043.19822316
ACGTCTG1002150.043.14914315
AGCACAC1005950.043.11874810
TCACGTC986350.043.06849330
CTCGTAT991750.042.97264544
CCGCACA972900.042.9615936