FastQCFastQC Report
Wed 4 Feb 2015
SE369_GTAGAG_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE369_GTAGAG_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15654450
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC6490944.146386490742248TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT756750.042.91780512
ACACGTC756900.042.91199513
GCACACG757500.042.89920811
GTATGCC734650.042.82310545
CACGTCT760200.042.67217614
CGTAGAG739700.042.47714633
AGTCACG752300.042.47354528
ACGTCTG763750.042.45910315
GTCACGT750150.042.3181129
AGCACAC770350.042.2507810
TCACGTA748750.042.25022530
CGTCTGA766600.042.2425616
GATCGGA763750.042.207231
CACGTAG751000.042.12677431
CGTATGC745000.042.1133744
CTCGTAT745650.042.08094842
CAGTCAC761650.042.01391227
TCGTATG749550.041.932843
TCGGAAG782450.041.9051133
AGAGCAC780100.041.840958