Basic Statistics
Measure | Value |
---|---|
Filename | SE369_GTAGAG_L006_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 15654450 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC | 649094 | 4.146386490742248 | TruSeq Adapter, Index 3 (97% over 37bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACACGT | 75675 | 0.0 | 42.917805 | 12 |
ACACGTC | 75690 | 0.0 | 42.911995 | 13 |
GCACACG | 75750 | 0.0 | 42.899208 | 11 |
GTATGCC | 73465 | 0.0 | 42.823105 | 45 |
CACGTCT | 76020 | 0.0 | 42.672176 | 14 |
CGTAGAG | 73970 | 0.0 | 42.477146 | 33 |
AGTCACG | 75230 | 0.0 | 42.473545 | 28 |
ACGTCTG | 76375 | 0.0 | 42.459103 | 15 |
GTCACGT | 75015 | 0.0 | 42.31811 | 29 |
AGCACAC | 77035 | 0.0 | 42.25078 | 10 |
TCACGTA | 74875 | 0.0 | 42.250225 | 30 |
CGTCTGA | 76660 | 0.0 | 42.24256 | 16 |
GATCGGA | 76375 | 0.0 | 42.20723 | 1 |
CACGTAG | 75100 | 0.0 | 42.126774 | 31 |
CGTATGC | 74500 | 0.0 | 42.11337 | 44 |
CTCGTAT | 74565 | 0.0 | 42.080948 | 42 |
CAGTCAC | 76165 | 0.0 | 42.013912 | 27 |
TCGTATG | 74955 | 0.0 | 41.9328 | 43 |
TCGGAAG | 78245 | 0.0 | 41.905113 | 3 |
AGAGCAC | 78010 | 0.0 | 41.84095 | 8 |