FastQCFastQC Report
Wed 4 Feb 2015
SE368_CCGTCC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE368_CCGTCC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16002823
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG209223413.074155728648625TruSeq Adapter, Index 16 (97% over 40bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG200790.12547161210243968TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTATG179380.11209272264024916TruSeq Adapter, Index 16 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG2454200.044.47366311
CACACGT2456300.044.40133712
ACACGTC2457100.044.36435313
CACGTCT2453900.044.35267314
TCGTATG2364200.044.3135845
ACGTCTG2458200.044.30437515
GATCGGA2426450.044.26781
AGCACAC2470500.044.25581410
CGTCTGA2461200.044.2302616
AGAGCAC2479200.044.1939778
TCGGAAG2479100.044.169683
GTCCCGA2349250.044.08894336
CGTCCCG2379600.044.08710535
CCGTCCC2387800.044.06117234
CCCGTCC2404800.044.03131533
GAGCACA2486850.044.010999
ACCCGTC2420700.044.0044432
GAACTCC2460250.043.99754321
CACCCGT2426050.043.9801631
GTCACCC2436750.043.9640129
Produced by FastQC (version 0.11.2)