FastQCFastQC Report
Wed 4 Feb 2015
SE367_ATGTCA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE367_ATGTCA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5814279
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG3912706.7294672305886944TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG452400.043.70952615
ACACGTC453050.043.62695313
TCGTATG447050.043.60671645
GCACACG453300.043.58341211
CACACGT453150.043.57264312
CACGTCT453450.043.50412814
CGTCTGA455300.043.42580416
GATCGGA456550.043.3090021
AGCACAC458300.043.24045610
TCGGAAG465700.043.1683433
CAGTCAC453200.043.12702627
CGGAAGA468350.043.090364
AGAGCAC464450.042.9778868
CTCGTAT451350.042.9768444
ACTCCAG453700.042.96965423
CCAGTCA453300.042.89874626
TCCAGTC454250.042.8387525
CTGAACT461650.042.8055919
GAACTCC458150.042.79034821
AGTCACA457550.042.7374128