FastQCFastQC Report
Wed 4 Feb 2015
SE366_AGTTCC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE366_AGTTCC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461278
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG1729207.025618398246765TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG195500.044.10602615
CACGTCT196350.043.9494614
ACACGTC197650.043.83111613
GCACACG198150.043.78863511
TCGTATG192350.043.7619945
CGTCTGA197000.043.7359316
CACACGT198600.043.6780912
AGCACAC199400.043.51413310
GATCGGA197900.043.412281
CGTATCT192950.043.3488139
CAGTCAC196300.043.3030127
CCGTATC192500.043.29730238
GTCACAG195000.043.2721429
CGGAAGA202050.043.2663044
TCGGAAG201600.043.265973
AGAGCAC201200.043.247838
CCAGTCA196250.043.22232426
TCCAGTC196900.043.148225
GAACTCC199150.043.14476821
TCCGTAT194100.043.1258837
Produced by FastQC (version 0.11.2)