FastQCFastQC Report
Wed 4 Feb 2015
SE365_TAGCTT_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE365_TAGCTT_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19656452
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC197920.10068958528222692TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC38050.024.36411531
CGTATGC39400.023.82218744
GTATGCC42100.022.56165945
ACTAGCT42850.021.7925832
GCACACG48550.021.50065611
ACACGTC49150.021.14610713
ACGTCTG49150.021.10028315
CTCGTAT46500.020.23224842
TCGTATG47500.019.90203743
CACGTCT52750.019.7029114
TAGCTTA49400.019.04098734
CACACGT55600.018.89579212
CAGTCAC52400.018.80563227
TCACTAG50750.018.79918530
CGTCTGA57350.018.20091416
CTAGCTT53600.017.80037133
ACTCCAG59500.017.6958223
CCAGTCA57300.017.31530626
GCTTATC54100.017.26142336
TCCAGTC60400.016.83629825
Produced by FastQC (version 0.11.2)