FastQCFastQC Report
Wed 4 Feb 2015
SE364_ACTTGA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSE364_ACTTGA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16729964
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC5259533.1437784325178466TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG613550.043.30603811
ACGTCTG612600.043.18880515
CACACGT615450.043.17913412
ACACGTC615550.043.1060813
CACGTCT615100.043.0205814
GTATGCC597850.042.9260845
CGTCTGA618150.042.78647616
CGTATGC599400.042.721244
GTCACAC610500.042.42770429
CTCGTAT604700.042.35653742
GAACTCC620850.042.34306721
GATCGGA619850.042.3042141
CAGTCAC616350.042.2488127
AGCACAC630200.042.2404110
TCGTATG607800.042.2010143
CCAGTCA617050.042.1899526
ACTCCAG621400.042.14404723
TCCAGTC619750.042.04582225
TCGGAAG639200.041.6967773
AGTCACA623350.041.6662228